Total polyphenol and anthocyanin contents and antioxidant
activity changes during storage
For determination of bioactive compounds (polyphenols and
anthocyanins) content and antioxidant activity, 100 mg of each
cookie was removed monthly, in triplicate. In order to extract
polyphenols from cookie samples 1 ml ethanol, acetic acid
and water (50:8:42) were mixed with 100 mg of ground cookie
sample on vortex for 1 min, then centrifuged at 6000 rpm for
3 min. Liquid part was collected and filtered through 0.45 lm filter.
Total polyphenol contents in cookie extracts were determined
by Folin–Ciocalteu method given by Singleton, Orthofer, and
Lamuela-Raventos (1999), total anthocyanins by Lee, Durst, and
Wrolstad (2005), while the antioxidant activity of cookie samples
was evaluated by spectrophotometrical DPPH assay according to
Tumbas Šaponjac et al. (2015). Results were expressed as mg gallic
acid equivalents per 100 g cookie (mg GAE/100 g), mg cyanidine-
3-glucoside per 100 g cookie (mg CyGE/100 g) and mM Trolox
equivalents per 100 g cookie (mg TE/100 g), respectively.
Sour cherry pomace extract encapsulated in whey and soy proteins:
Incorporation in cookies